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Sample extracts are then transferred to new nuclease-free 0.2 ml microvials (MidWest Scientific) and the desired final volume of 36 μl for each sample is additionally ascertained during this final sample transfer (or samples are used right away in the real-time RT-PCR application and not transferred).

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That is, additionally, absurd.

Spearman's non-parametric correlation tests were additionally carried out to ascertain correlations between age and miRNA expression levels.

To ascertain that HF-Doa1 binds to MBP-Wss1 all bound fractions were additionally analyzed by western blotting with α-His6 antibodies (panel B ). (C ) Interaction of Wss1 with preformed Cdc48 complexes confirms ternary 1 1 1 Wss1/Doa1/complexomplex.

To ascertain OTR involvement in OT-GKR-mediated cardiomyogenesis, OT-GKR-treated EBs were additionally exposed to 10−6 M OTA (Fig. 3F).

Additionally, it is ascertained that the hydrothermal synthesis method is able to produce a spherical shaped submicron particle.

Additionally, frailty was ascertained prior to the report of falls, so its predictive value could be assessed.

Additionally, dietary information was ascertained using a validated food frequency questionnaire, and information was available on several potential confounders.

Additionally, delivery weight was ascertained 2 4 weeks prior to delivery for 3.9% of the women with measurements in the EHR (31.36 ± 5.88 kg/m) and is likely an underestimate of their true weight at delivery (for women measured within 2 weeks of delivery: 32.72 ± 5.84 kg/m), as is delivery weight by self-report (31.74 ± 5.72 kg/m).

Unilateral or bilateral disease was also ascertained.

Smoking status was also ascertained.

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