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Both at the behavioral and at the Purkinje cell level, the old response is extinguished and the new response at the new time is acquired separately.
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Positive and negative mode data were acquired separately.
MRI and 18F-choline PET/CT were acquired separately on average 21 days apart (range 0 47 days).
The performances of the fabricated EDFRR were measured and the experimental Q-factor of 2.47×108 and resonant depth of 109% were acquired separately.
For region of interest identification, a T1-weighted MR image was acquired separately with a Siemens 3 T Trio scanner using an extremity coil and the following spin echo sequence: [echo time (TE) = 3.34 ms, repetition time (TR) = 2530 ms, flip angle = 7°, thickness = 0.50 mm, field of view = 140 mm].
Absolute thresholds were acquired separately for 1 kHz and 0.5 kHz pure tones and broadband noise using a 1-up 1-down adaptive staircase before any of the noise conditions were run.
To study mutual orientation of the GA and the initial contact expansion, cells were pre-incubated with the GA fluorescent probe brefeldin-BODIPY558 (Molecular Probes) at 0.1 µM for 20 min. Three-dimensional images were acquired separately on the wavelength corresponding to the fluorescence of the GA label, and in transmitted light showing the area of cell contact with the substrate.
For multicolor fluorescence images, each channel was acquired separately.
In contrast, several hydrolases appear to have been acquired separately by one or two Frankia strains.
Images were acquired separately for all four fluorochromes using appropriate excitation and emission filters (AHF Analysentechnik).
However, to calculate this parameter of LV dyssynchrony, three apical views need to be acquired separately.
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