Plasma levels of chemokines and vascular cytokines were assessed by MesoScale technology, which is a multiplex type of ELISA technology that relies on electrochemiluminescence, according to the manufacturer's protocol.
Our real-time RT-PCR is a multiplex assay that employs a mixture of 2 sets of inhouse designed primers and dual-labeled fluorescent probes that specifically target 2 different regions of the HA gene of H5N1.
Another approach would be a multiplex array comprising of antisera for all known PIDs.
Library 3 was a multiplex sequencing library of three equimolar indexed samples, which was enriched using one target enrichment reaction.
Library 4 was a multiplex sequencing library of nine equimolar indexed samples, which was enriched using one target enrichment reaction.
As compared to FISH, MLPA has the advantage of being a multiplex technique in which very small (50 70 nt) sequences are detected.
The EUROFORGEN Global ancestry-informative SNP (AIM-SNPs) panel is a forensic multiplex of 128 markers designed to differentiate an individual's ancestry from amongst the five continental population groups of Africa, Europe, East Asia, Native America, and Oceania.
For parentage analysis, parents and offspring were genotyped with a multiplex of nine microsatellite markers, from which six (CL2416Contig1, CL120Contig1, cDN14P0004O13.F.ab1, CL2598Contig1, CL2595Contig1, cDN08P0004J06.F) were newly developed and the other three (Dd67, Hd46, Eld36) were from RH 18 (Fig. 1).
Fragment analysis genotyping was performed using a multiplex of primers, including one standard primer and one M13-tagged primer per marker primer pair, plus one fluorescently-labeled (PET™, NED™, VIC® or 6-FAM™ dye; Applied Biosystems) M13 primer [ 39].
Spoligotyping is a form of multiplex PCR in that multiple loci must amplify to obtain a complete fingerprint and multiplex PCRs tend to be less sensitive than those amplifying single loci [ 37].
