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Genomic sequences were useful for identifying full-length sequences, irrespective of transcript abundance, as well as for providing the intron-exon structure of the gene.
Our finding that older animals, in general, have higher coefficients of variation, irrespective of transcript length, confirms that age-related variation is genome-wide.
Our finding that dinucleotides were the most frequent repeat motif in white shark transcripts irrespective of transcript region is typical for a wide range of taxonomic groups including other vertebrates [ 41].
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As such, H3K4me2 marked 84% of genes (Table 1) irrespective of the transcript levels produced from these genes.
For example, background levels of hybridization (i.e. hybridization to a probe that occurs irrespective of the corresponding transcript's expression level) limit the accuracy of microarray expression measurements, particularly for transcripts present at low abundance.
As shown in Figure 5, irrespective of the fusion transcript abundance all three tools detected EWS-ATF1, two tools detected EML4-ALK, and only one tool detected TMPRSS2-ETV1.
Irrespective of whether the transcripts were rare (Gs and Pdk4) or abundant (Pepck and UbC), their changes in expression were similar in the microarray and qPCR quantifications.
If ≥2 exons mapped to the same transcript, we reasoned that the entire mRNA transcript (irrespective of isoform) was differentially expressed.
The high variability in the low abundance region of both amplified and non-amplified experiments show that this is in an unreliable region of transcript concentration irrespective of the inclusion of an amplification step [ 34].
Leaves from wild type controls (AtSUC2 +/+) and from the representative AtSUC -/- lines, 1039 (SUC2p::cSUC2), 1070 (CoYMVp::cSUC2), and 1133 (rolC::cSUC2) accumulated [C] to similar levels, irrespective of differences in SUC2 transcript abundance, growth, and transient carbohydrate levels (Fig 4A).
Put another way, if such a putative transcript exists and induces the observed long-range correlation between gene expression signals by binding to multiple probe sets, it cannot be a typical protein-encoding transcript, irrespective of whether its affinity to the probe sets is high or low.
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