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Menstrual irregularities were defined as oligomenorrhea (eight or fewer menses/ year) or amenorrhea (absence of menstruation for 3 consecutive months).
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RPE irregularity was defined as a condition including a small RPE bump, RPE hypertrophy or atrophy.
US erosions were defined as irregularities of the bone surface of the area adjacent to the joint and seen in two planes, as suggested by Szkudlarek and coworkers [ 31].
Osteophytes were defined as a definite irregularity in the bone cortex of the femoral head or neck, while femoral head abnormality was present if there was flattening or loss of contour of the femoral head.
Large-amplitude ESF irregularities are defined to be the irregularities with ΔN > 2 × 1010 m−3, and small-amplitude ESF irregularities are those with ΔN in the range of 1 2 × 1010 m−3.
Tregs were defined as CD4+CD25+Foxp3+.
Apoptotic cells were defined as hypodiploid cells.
DC were defined as ILT-3+/CD14+/CD16− cells.
Macrophages were defined as F4/80+ cells cells.
Behaviours were defined as previously described [37].
The two groups were compared by patient's satisfaction, improvement of the irregularity index (Little's Irregularity Index is defined as the summed displacement of adjacent anatomical contact point of the six mandibular anterior teeth [11]), speech impairment, and mean wear time.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com