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hESCs display a higher percentage of cells with RAD51 foci at early times after irradiation when compared to NPs, indicating more robust HRR, whereas in astrocytes, no RAD51 foci were seen.
Moreover, the overexpression of Aurora-A substantially aggravates the effect of irradiation, when compared to cells expressing GFP alone.
Statistical analysis confirmed, that synergistic effects are more pronounced and occur with greater likelihood after sequential application of TRAIL after irradiation when compared to concomitant treatment schedules.
Doxorubicine did not show a significant additional effect with concurrent irradiation when compared to individual substance treatment (Additional file 5), in agreement with the response of monotypic 3D-microtissues (Additional file 3).
In the study of Aoyama et al. neuro-cognitive function was better in patients treated with radiosurgery plus whole-brain irradiation when compared to patients treated with radiosurgery alone at one year and two years after treatment [ 8].
Moreover, all peak myocardial velocities (S′, E′, and A′ velocities) of different segments of LV posterior wall and RV free wall using color coded TDI were significantly lower in the group exposed to both anthracyclines and mediastinal irradiation when compared to the group exposed to anthracyclines only.
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Although both treatments resulted to progression of cell cycle to S phase, only pre-treated HDFs showed decreased induction of apoptosis while no changes was observed in HDFs treated during-irradiation when compared to irradiated HDFs.
This observation is in line with the previous report that LCs are more resistant to irradiation injury when compared to GCs.
Also, please note that the experiments in current Figure 4 figure supplement 3 applied lower irradiation doses when compared to original Figure 3A (now Figure 4B).
In conclusion, we confirm the dosimetric advantages of SIB for breast irradiation, even when compared to an advanced and highly conformal sequential technique.
The time required for the etching process using excimer irradiation is shorter when compared to the time taken without irradiation under similar processing conditions.
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