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All the canine retraction (and laser irradiation) procedures were performed at evening sessions (between 17 and 20 o'clock).
Analogous irradiation procedures have been carried out at room temperature (RT) and at T = 620 K (≈0.9 Tg, where Tg denotes the glass transition).
The characterization of UVB contribution to the lethal effect of sunlight on bacteria is relevant for understanding of the mechanism of cell death, and for improvement of dosimetry techniques and irradiation procedures.
It has been demonstrated that by utilizing the LINAC, the internal irradiation procedures has been proven to be safe and feasible, and along with the significant cost/time reduction suggested that it is more advantageous than external procedures in hospitals without dedicated devices [22].
SH performed all the irradiation procedures.
KAC assisted with irradiation procedures and provided consultation on data analysis.
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For irradiation of attached cells, cells were plated in culture dishes on Day 1, and irradiated at 70 80% confluency on Day 2. All experimental samples in dishes were kept rotating on a turntable, inside the irradiator machine, during the irradiation procedure.
The solution was stirred continuously during the irradiation procedure.
The response and recovery time obtained without any heating to facilitate desorption of gas species and UV irradiation procedure.
Kinetic studies have also been performed in order to follow the evolution of nanospecies during irradiation procedure.
The radiation assays were conducted on ice (4 °C) under atmospheric conditions, and the cell population remained constant during the irradiation procedure.
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