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Decreasing the Mw of alginate using irradiation (e.g., irradiating at dose of 2 Mrad) decreased the low shear viscosity of 2% (w/w) pre-gelled solutions from 1000 to 4 cP, while maintaining high elastic moduli, once cross-linked to form a gel.
These novel structures were obtained by irradiating at various angles (+30°, −30°).
Photocurrent-voltage (J-V) was measured by using a Keithley 2400 source/meter controlled by a PC, while irradiating at 100 mW · cm−2 (1 sun) with AM 1.5G simulated sunlight produced by a class 3A solar simulator (Newport, 94043A, Irvine, CA, USA).
Feeder cells were prepared from spleens of naïve syngeneic mice by pulsing with 0.5 uM peptide for 1 hour at room temperature and irradiating at 3,000 Rads before plating.
As expected, the release of fluorescein is faster at higher CW power, with an on/off ratio of 18 when irradiating at 350 mW compared to 14 and 5 when irradiating at 260 and 170 mW, respectively.
Plates were then irradiated for 30 min on a UV Stratalinker 2400 (Stratagene) containing bulbs irradiating at 254 nm.
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The transfected DCs were washed and irradiated at 30 Gy γ-irradiation using γ-cell irradiator Gamma-Elite10000).
Petri dishes coated with Matrigel were irradiated at 20 Gy using a Co irradiator.
After 24 h the cells were irradiated at 0.75 Gy using a Co irradiator (Theraton, Phoenix, USA).
Moreover, irradiation of cells incubated with unlabeled TAT and irradiated at the TMR excitation wavelength did not die (Fig. 2A).
Cells were irradiated at the indicated doses using a Co irradiator.
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