Calculated strength factors were successfully used to estimate radiation hardening using the broadly employed dispersed barrier-hardening model, leading to good agreement with experimentally measured hardening in neutron-irradiated type 304 and 316 stainless steel variants.
However, when we transplanted BM cells from the RBP-J deficient and the control mice into irradiated wild type mice, we did not observe significant difference in PB EPC or CXCR4 level between the RBP-J deficient group and the control group, suggesting that the mobilization of EPC into PB needs other signals.
On the other hand, it seems unlikely that an enhanced direct interaction between stromal fibroblasts and tumor cells could account for the earlier tumor establishment in D849N-mutant mice, since there was no difference in proliferation of B16 melanoma cells co-cultured with either irradiated wild type or irradiated D849N-mutant mouse embryonic fibroblasts in vitro (data not shown).
In adult zebrafish, the site of hematopoiesis is in the kidney, and dmPGE2 was shown to be effective at stimulating HSC dependent kidney marrow recovery in irradiated wild type fish [ 59].
Herijgers et al.[ 129] transplanted bone marrow from Ldlr−/− mice into irradiated wild type mice and, despite no significant changes in serum cholesterol or lipoprotein profiles, animals developed atherosclerotic lesions.
As an alternative approach, Chan et al. examined the influence of SHP-2 haploinsufficiency upon HSC function in competitive repopulation studies wherein heterozygote ptpn11 +/− and wild-type HSCs were co-transferred into irradiated wild-type recipients (Chan et al., 2006).
The lack of autoantibody formation was not due to an absence of alloreactivity, because spleen cells from bm12 mice proliferated equally in response to irradiated wild-type and Mer-KO irradiated spleen cells.
Transplantation of wild-type and Mtgr1-null bone marrow into irradiated wild-type mice demonstrated that the severe DSS-induced ulceration seen in Mtgr1-null mice was due to a colonic, rather than a hematologic, defect.
Lmna-/ bone marrow reconstituted normal T and B cell development in irradiated wild-type recipients, driving generation of functional and self-MHC restricted CD4+ and CD8+ T cells.
Endometriotic lesions were implanted into irradiated wild-type mice transplanted with GFP-expressing bone marrow.
Following dissection and manual dissociation of two SRBCTs derived from tp53 mutants, approximately 1 million cells were injected intraperitoneally into irradiated wild-type adult zebrafish.
