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We observed that while un-irradiated ECs exhibited limited and sporadic CD44 staining, irradiated ones expressed very high levels of this protein (Fig. 4A), with some cells expressing much higher levels than others (Fig. S6).
Moreover the smear layer in NaOCI irrigated teeth was excessive to those with laser irradiated ones.
Compared to the unirradiated tumors, we observed many more large fenestrae on the endothelial walls of the irradiated ones.
A clear color change was detected with respect to the unirradiated areas; the irradiated ones appear black (not shown here, see Additional file 2).
Cells that have not been directly exposed to radiation can receive signals from irradiated ones that were in the vicinity responding in a similar way to direct exposure (Najafi et al. 2014).
Those control samples were made of sham-irradiated cell cultures incubated in parallel with the irradiated ones for 4 h.
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Samples were irradiated one-by-one for 3 min in the Hoger Onderwijs Reactor in a thermal neutron fluence rate of 1.7 × 10 m−2 s−10
Intravenously injected lineage negative-enriched bone marrow from young mice into sublethally irradiated one month old and 18 month old mice showed absolute number of donor cells was similar in young and older hosts after three days [ 31].
Samples were then irradiated on one side only with UVA light (365 nm at 2×1017 photons s−1 for 24 h), producing asymmetric photodegradation of the pigmented polymer layers.
The laser handpiece was fixed, and the plate was moved to irradiate one well at a time (5 cm above the bottom of the culture plate).
Media were changed and one plate irradiated (with one as a dark control).
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