Dry Arabidopsis thaliana seeds were irradiated with carbon (C) ions with LETmax at a dose of 400 Gy and with LET of 22.5 keV μm-1 at doses of 250 Gy or 450 Gy.
The tribological and physico-chemical properties of electron beam irradiated POM-C blocks have been analyzed using pin on disk tribometer, Raman spectroscopy, FTIR-ATR, gel content analysis, SEM-EDS (scanning electron microscopy-energy dispersive spectroscopy), surface profiler and contact angle analyzer.
In the present investigation, we studied nontransfected human glioma cells irradiated with Cs- γ rays at different absorbed doses (20, 40, 60, 80, 100 and 120 Gy).
As a positive control for genotoxic effect, the cells were irradiated with Cs γ-rays, at 3 Gy, using a Gammacell 1000 source (Atomic Energy of Canada Limited, Ottawa, Canada).
Although the response of individual specimens to radiation exposure varied, as expected, the cultures irradiated with Cs γ-rays contained significantly more SA-βgal and BrdU cells, as in the X-ray experiments, than unirradiated controls (P < 0.0001; Wilcoxon test).
To test for differential sensitivity to γ-rays and UV light, cells were plated in culture medium, irradiated with Cs γ-rays or UV, and then incubated at 39.5°C for 48 hr.
As a positive control for genotoxic effect, the cells were irradiated with Cs γ-rays (3 Gy) using a Gammacell 1000 (Atomic Energy of Canada Limited, Ottawa, Canada) source at 10.6 Gy/min.
The next day, cells were washed with PBS and irradiated with 100 J/m2 UV-C (254 nm, UV Stratalinker® 2400, Stratagene) after removal of the PBS.
These vials were irradiated using a Cs irradiator with an average dose rate of 6.5 Gy/min.
Cells were irradiated with a Cs irradiator (JL Sheppard, San Fernando, CA, USA) at the University of Medicine and Dentistry of New Jersey Newarkk, NJ, USA).
