In this study, we demonstrate that passive transfer of immune sera protected irradiated mice against subsequent infection with strains of F. tularensis subsp. holarctica.
To investigate IFN-γ expression of human T cells against alloantigen, we cultured the splenocytes for 6 hours at a density of 1×106 cells in 96-well plates with the irradiated PBMC or irradiated self-CB.
All of these mechanisms described above for enhancing the immune system activity against tumors act locally against tumors being irradiated, but a local immune response can also translate into systemic immunity, inducing "abscopal" effects at tumors distant to the irradiated site [ 24].
Lethally irradiated MK16/1/IIIABC cells induced protection against challenge with 10homologous cells, but not against a higher cell dose (5 × 10).
Untreated or anti-CD16 antibody treated irradiated monocytes did not mediate cytotoxicity against hMSCs, (data not shown).
As urocanic acid is known to protect skin against UVB radiation, we irradiated shc- and shFLG2-RHE and detected active caspase-3 as a marker of apoptosis, as previously described.
By contrast, only 2 of 44 volunteers immunized by i.d. or s.c. inoculations with variable numbers and doses (7500 × 4 to 135,000 × 6) of purified cryopreserved irradiated P. falciparum sporozoites were fully protected against challenge with infectious mosquitoes (Epstein et al, 2011).
As a safety precaution against infection, culture supernatants were UV irradiated for one hour to inactivate residual virus prior to ELISA analysis.
Aqueous solutions of 5-substituted hydantoins were irradiated with ultraviolet (UV) light, to investigate their structural stability against UV radiation as well as the possible photolysis products.
