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The unique iron coordination of IsdB is predicted to be important for its unique role as a Hb receptor and initiator of heme import by the Isd pathway.
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These members share a common catalytic center in heme by iron coordination to the thiolate of a conserved cysteine [ 68].
Table s4 in Additional file 1 lists the Hr sequences found to deviate from the canonical Hr sequence, either through alteration of one or more residues involved in iron coordination or loss of a helical segment: 58 out of the 327 bacterial sequences (18%) in 34 genomes, and one annelid.
This observed increase in entropy is consistent with that demonstrated in a prior metal binding study with cPAH from which it was concluded that discharge of water from the active site leading to iron coordination is the cause of the increased entropy upon metalation.
XPS results provide evidence of the iron coordination with TPP molecules.
These data suggest that the increased levels of heme generated from the addition of ALA drives the substrate interaction dynamics of the HCCS mutants with heme-binding defects (i.e., defects that are independent of the heme iron coordination chemistry).
In comparison, the RFQ-RR spectrum of the intermediate in H83A supports iron coordination by an O atom, either from solvent or from Tyr75, before coordination of the second axial ligand, His32.
Figure 3 B shows the optical response of TDPP before and after iron coordination.
In the first section, the description of chemical environments encountered in this iron coordination chemistry is briefly introduced.
The iron coordination in SyrB2 is unusual, with only two histidine ligands coordinating the iron.
(10) Moreover, it has been shown that reduction of the heme-iron in IsdA results in heme iron coordination solely by the distal His83.
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