Sentence examples for ip kit from inspiring English sources
M-PER cell lysates were subjected to immunoprecipitation (IP) using either the Profound Mammalian HA Tag IP/Co IP Kit or the Profound Mammalian Myc Tag IP/Co IP Kit, as per manufacturer's specifications (Pierce Biotechnology).
The ChIP assay was performed using a SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology) in accordance with the manufacturer's instructions.
For SETDB1 ChIP-seq, ChIP samples were prepared as for ZNF274 and KAP1 from 108 K562 cells, using 20 µg anti-SETDB1 antibodies (Proteintech Group, catalog # 11231-1-AP), exchromatinmatin was prepared using the SimpleChIP™ Enzymatic Chromatin IP Kit according to the manufacturer's recommendations (Cell Signaling Technology, catalog # 9003).
Lysate was centrifuged at 10,000 g for 5 min at 4°C, and extract volumes containing equal amounts of wild-type and each mutant Nkd1 protein (as determined by quantitating a western blot with loaded with equal volumes of total protein extract) were immunoprecipitated (IP'd) using the M2 α-Flag IP kit (Sigma-Aldrich).
Protein immunoprecipitation was performed with IP kit (Pierce, Rockford, IL, USA).
ChIP was performed using the SimpleChIP Enzymatic Chromatin IP Kit (Cell Signaling Technology) as previously described.
Equal amounts of protein (500 ug) from each sample were subjected to immunoprecipitation (IP) with mouse anti-SUMO-1(GMP1, Invitrogen, Carlsbad, CA, USA) using nuclear complex co-IP kit (Active Motif, Carlsbad, CA), and then subjected to SDS-polyacrylamide gel electrophoresis.
Immunoprecipitations: for Myc-tagged SIRT1 constructs (Figures 5B and 5D), Mammalian c-Myc Tag IP/Co-IP kit from Pierce (#23625) was used.
35S-labeled Myc-tagged TbUNC119 and 35S-labeled HA-tagged TbUNC119BP were immunoprecipitated using the Matchmaker Co-IP kit (Clontech) according to the manufacturer's protocol.
The co-IP complexes were recovered using EZview™ Red ANTI-FLAG® M2 Affinity Gel (Sigma-Aldrich) or ProFound™ HA Tag IP/Co-IP Kit (Pierce) according to the manufacturer's instruction.
For mammalian cells, the co-immunoprecipitation of HA-tag proteins was carried out with ProFound Mammalian HA Tag IP/Co-IP kit according to manufacturer's instructions (Cat.No. 23615, PIERCE).
