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The eluting peptides were ionized by a Finnigan nanospray ionization source (Thermo Scientific, Basel, Switzerland).
For this purpose a pneumatically assisted electrospray ionization source with a new design was constructed.
The electron impact (EI) ionization source was operated under 12 eV ionization energy.
The atmospheric pressure photo ionization source was purchased from Bruker Daltonics.
The quadrupole and ionization source temperature were 150°C and 280°C, respectively.
The mass spectrometric detection was carried out using an electrospray ionization source in positive mode.
Improvements in detection limits, by as much as 100×, are anticipated with improved ionization source designs.
The mass spectrometer was equipped with an electrospray ionization source and operated in positive polarity.
The quadrupole, ionization source temperature were 150°C and 280°C, respectively.
It should be noted that direct meteoric ionization is the only ionization source considered here.
To that end, an appropriate sample introduction system with a novel design was constructed for the paper spray ionization source.
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