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Using gradient ionic detergent, we optimized the preparation procedure for the decellularized liver biologic scaffold, and analyzed its immunogenicity and biocompatibility.
Results in Fig. 2a indicate that the presence of β-ME and high concentration of ionic detergent (SDS) favors the detection of the Hv1 monomer.
GSL fractions were dissolved in non ionic detergent (1% TritonX-100) to maintain some protein interactions and heated for 1 hour at 37°C to allow extraction of proteins from membrane cholesterol.
The Hv1 dimer is stabilized, at least in part, by strong polar interactions in granulocytes, because high concentration of ionic detergent (4% SDS) was required to efficiently disrupt them (Fig. 2a).
Membrane proteins isolated from VF12 strains were solubilized prior to gel-filtration chromatography using either non-denaturing detergent perfluorooctanoate (PFO), which preserves interactions within protein oligomers [23], [24], or with SDS, a strong ionic detergent which disrupts protein interactions [25].
The collected sperm were sonicated in Triton X-100 to remove tail and plasma membranes, plus ionic detergent treatment to produce demembraned sperm heads for microinjection [13], [36], [68].
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This somewhat harsh protocol, which employs two ionic detergents and one non-ionic detergent, yields highly regular arrays of junctional channels retaining the characteristic double-membrane structure.
However, Tween-20 is a non-ionic detergent, but not all detergents are non-ionic: SDS and urea for example are ionic detergents (also known as chaotropic agents).
Non-ionic detergent was used to remove the membrane of purified herpes simplex virus under control and reducing conditions.
The PET substrates were sequentially rinsed in four ultrasonic baths: non-ionic detergent, de-ionized (DI) water, acetone, and isopropyl alcohol, each for 10 min, respectively.
In this regard, we found out that using 0.3%% w/v of non-ionic detergent, which primarily involves the removal of fat and soluble proteins was optimum.
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