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The solid-supported membrane assay with nanodisc samples provides reliable control over the ionic condition and information of the relative ion activity of this promiscuous pump.
By varying the ionic condition (i.e., pH and Mg/Ca ratio) of the employed salt solution, calcite (Cal), aragonite (Ara), and solely amorphous CaCO3 (ACC) were separately deposited inside the EC/PAA matrices.
Oxide film growth occurs by an activation-controlled ionic condition under the influence of a high electric field across the film according to an exponential law as on valve metals.
The binding affinity of MREs is highly dependent on their three-dimensional structure and is influenced by factors including the ionic condition, temperature, and pH of the binding condition [ 4].
The fact that freshwater fish blood has maintained a sea-water like ionic condition is precisely the point, as it demonstrates the necessity of maintaining correct extracellular ion concentrations for cell survival.
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Experiments in outside-out configurations were performed in symmetrical ionic conditions and in a divalent-free buffer.
Using these ionic conditions, we repeated the experiment in Fig. 2e, and as expected obtained inward currents at P4 at the +40 mV holding potential (Fig. 2g, i).
These experiments demonstrate that under approximately physiologic ionic conditions only a small percentage of subunits in highly purified skeletal muscle or Dictyostelium F-actin participate in exchange.
Actin filaments, assembled from highly purified actin from either skeletal muscle or Dictyostelium amoebae, are very stable under physiological ionic conditions.
We report here that the unmodified DNA polymer d TG n·d CA)n readily undergoes a transition to a Z conformation when subjected to unwinding torsional stress in ionic conditions that are close to physiological.
Our strategy is complemented with flash photolysis data, where the lifetimes of different photointermediates were determined at different ionic conditions.
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