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At a holding potential of −70 mV, CIN stimulation evoked an average (n = 7) inward peak current of 16.7±4.4 pA (Fig. 2C), estimating the contribution of AMPARs.
We estimated the peak ICaL by the difference between the inward peak and the current level at the end of a 500 ms pulse from Vh −50 mV to 0 mV, where the maximal ICaL occurred.
Voltage-dependent Na+ and K+ currents were measured after leak subtraction using a p/−6 protocol and automatic detection of the fast inward peak and the late outward plateau.
Originally, N143S currents were described as not noticeably different from the wild-type TRPC6 peak channel activity.(10) In this study, the outward peak current of the N143S mutant again was not significantly different from the WT current, but caused a significant increase in the inward peak current amplitude (Figure 3B).
Inward peak current amplitudes were measured.
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Similarly, 45% of these cells exhibited a fast inactivating inward current (peak current=201±30 pA) in response to application of 100 μM AMPA (n=11, Figure 3m).
Peak inward current induced by the test compound were normalized to the peak amplitude of current evoked by 20 µM carbachol.
A summary of 12 such experiments (Fig. 5 F ) shows that the mean peak inward current increased in amplitude and that the voltage at which current peaked shifted negatively by 10 mV.
An increase in the magnitude of the peak inward currents was also noted.
Ankle kinematics data were collected by a tri-axial gyroscope motion sensor and the peak inward heel tilting velocity was obtained to represent the effect in resisting the simulated ankle spraining motion.
The fitting region extended from baseline to 10 mV beyond the peak inward current.
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