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The production of our candidate 2015.0 provisional IGRF model involved several steps, all of which are detailed below ("Methods" section).
The preparation involved several steps such as changing the pH value of the suspension, using surfactant activators, and using ultrasonic vibration.
Our previous protocol for producing the protein from Escherichia coli involved several steps with low overall yield: expression as a fusion protein, denaturation and renaturation, oxidation of the cysteines, and cleavage by cyanogen bromide at an engineered methionine adjacent to the desired N-terminus.
The subcloning strategy involved several steps.
Initially, a new design that considers the blocker and biosensor domains as distinct entities was performed by including them at each end of the P2 stem of the HDV ribozyme in a process that involved several steps (Figure 1B).
While our strategy involved several steps and training data were used repetitively to refine the set of the most informative assays it is critical to appreciate that only the model development set, composed of the stage I data and an equal number of non-ovarian cancer data, were used repetitively.
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This involves several steps: ascertain which banks are insolvent, take them over, sever the most toxic assets and sell them over time or hold them to maturity.
It would involve several steps.
But the procedure can be complicated, involving several steps and requiring some training and practice.
This translation involves several steps, most of which are normally done by dedicated signal-processing chips.
The conversion of purified titanium sponge to a form useful for structural purposes involves several steps.
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