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We found no significant changes between groups, suggesting other mechanisms may be involved (data not shown).

Phylogenetic analysis did not provide any convincing evidence for gene transfer for any of the genes involved (data not shown).

In earlier experiments we observed these "mixed fragments" at frequencies between 0.1 and 16% of all obtained reads per run with higher frequencies when more than two samples were involved (data not shown).

Moreover, neither oxidative stress nor cathepsins - which have been previously implicated in SphK1 activity inhibition caused by other stimuli in various cell systems [49], [50] - seemed to be involved (data not shown).

Control experiments confirmed that the reaction is insensitive to catalase, implying that H2O2 is not involved (data not shown).

Sequencing of cDNA from an 851del4-homozygous pandenthend the p.R605Q-heterozygous individual revealed correct splicing of the exons involved (data not shown).

The significant association between nodal status and PTSS was largely unaffected when adjusting for whether the women had undergone sentinel node procedure and the number of lymph nodes involved (data not shown).

However, when cells expressed the CAPS-associated mutant NLRP3 with the Tet-on system, a cathepsin B inhibitor blocked both cell death and mature IL-1 β release (Supplementary Figure S5c), even though a formation of large particles and lysosomal rupture was not involved (data not shown).

In our study, the surgeons involved did not show a preferred table position, when asked.

Further addition of any other SNPs involved did not show significant improvement of the model, which is partially explained by the high LD among most of these SNPs.

Transfected Irgm1 also co-localised with LAMP1 positive compartments in unstimulated cells taking up latex beads (Figure 9A arrows), as well as in cells not involved in phagocytosis (not shown).

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