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Of the total 17 significant tests detected in the subsamples nine involved locus CxpGT9, that also showed significant heterozygote deficits.
There were 21 and three isolates missing a significant portion of one or two loci, respectively, and 11 of these cases involved locus 5439.
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Moreover, additional regions have been recently implicated in the disease, which would increase the number of involved loci.
An obvious requirement for the occurrence of the accelerated evolution through cooperation/cheating is that the m-hit mutant does not arise almost instantly during sequential evolution, which would occur if the mutation rate were too high relative to the number of involved loci.
The remaining eight interactions involved loci which were individually nonsignificant.
The remaining four interactions involved loci that were individually nonsignificant (Table S2).
Among all traits, only two of the 17 interactions involved loci that both had significant main effects.
Among all traits, only one of the five interactions involved loci which both had significant main effects.
Single-locus-based methods usually are unable to recover all involved loci, especially when individual loci have little or no marginal effects.
In contrast, when the response variation reflects the combined effect of multiple genes, a continuous frequency distribution of the outcome variability may be seen due to the presence or absence of many small contributions from the involved loci.
In healthy adults (post-menopausal women), most age-related changes involve locus hypermethylation and are not associated with the disabilities that variably accompany ageing (16).
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