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All chosen in silico SNPs involved EST sequences from at least two different cDNA clones and had a probability of occurrence of 0.95 or more, according to the statistical assessment conducted with a bayesian method [ 18].
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He cited a case involving EST of Hawaii, which was denied tax exemption after the United States Tax Court found that it "was simply the instrument to subsidize" for-profit companies that would have provided it with a variety of services and "had no life independent of these corporations".
There are several previous studies involving EST approaches that have been carried out in Trichoderma species.
*Unigenes derived from a consensus sequence involving ESTs from different species are indicated as contigs, while singletons are listed by species.
saying I was representing a group of ex-est members, which, in part, was true because (some) people who were involved in est went to Scientology.
S HB and EMS were also involved in EST sequencing.
Furthermore, in order to improve sequence annotation and to gain insights into the cellular processes where each sequence could be involved, the ESTs were submitted to GOblet [ 40] and AmiGO [ 39] web servers.
In addition to the AS genes and events identified by mapping ESTs to the genome, using the BLASTN method implemented in ASFinder, we also identified 56 genes (involving 129 ESTs) from 7298 ESTs, which were not mapped to the genome.
TIGR gene index construction involves comparing EST and gene sequences using FLAST (Quackenbush et al., 2001).
Given the number of ESTs involved and the total number of ESTs in both pools, the differential expression of the contig in the specific tissue is evaluated.
Galactomannan hydrolases were the most abundant class of ESTs involved in galactomannan metabolism in the seed EST libraries.
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