Sentence examples for involved affinity from inspiring English sources

Proteomic characterization of kinetochores has involved affinity purification of proteins that associate with kinetochore components such as CENP-A [ 72 74] or CENP-S [ 75].

Standard mRNA purification and cDNA synthesis procedures used for cell populations (∼>10000 cells) involve affinity columns, and wash and precipitation steps that are not suited for the processing of samples derived from single cells.

The recombinant proteins were soluble and purified through four subsequent chromatographic steps, as described in Materials and Methods, involving affinity chromatography (Ni-NTA), anion- (MonoQ) and cation- (MonoS) exchange chromatography and hydroxyapatite (HAP) column.

Many of them involve affinity chromatography using an in vitro transcribed-RNA bait.

The next step involves affinity purification of mRNAs using oligodT, instead of trimming down the RNAs as performed in CLIP-type experiments.

Moreover, we predicted one out of three interactions for Cbf11 that were produced as hits from an experimental approach involving affinity (TAP) purification followed by mass spectroscopy.

To identify novel components that mediate PtdIns 3,4,5) P3 signaling in chemotaxis, we used a proteomic approach involving affinity purification of PtdIns 3,4,5) P3-binding proteins from Dictyostelium cytosol and their identification using mass spectrometry.

The composition of the MID1 protein complex was determined by several independent methods: (1) yeast two-hybrid screening and (2) immunofluorescence, (3) a biochemical approach involving affinity purification of the complex, (4) co-fractionation in a microtubule assembly assay and (5) immunoprecipitation.

Is it a good idea to get involved with affinity groups — for example, a group representing African-American women or Latino men? A. Affinity groups can help you personally and professionally as long as the group doesn't exist solely as a way for members to meet and socialize with one another.

This involved an affinity capture of the cells using anti-CD66B antibodies, and stimulation with either bacterial lipopolysaccharide (LPS), or granulocyte-macrophage colony-stimulating factor (GM-CSF) plus interferon (IFNγ).

This N-terminal fragment was observed with similar abundance even when the purification procedure was modified to limit proteolytic cleavage of the recombinant protein (the modification involved performing affinity chromatography at 4°C and increasing the concentration of protease inhibitors in the bacterial crude extract).