GSB conceived of the study, supervised the molecular biology activities involving protein expression, and antibody humanization, and contributed to writing the manuscript.
To further confirm the role of HER2 in regulating HIF-1α and BCRP and to determine if ERα is also involved, protein expression in Hc7 cells, ERα + MCF-7 cells transfected with HER2 gene was also studied.
Phenotypic characterizations have been reported elsewhere and involved protein expression patterns of the cell lines for the breast cancer subtyping (cytokeratins, ER, PgR and ERBB2) and expression of the intrinsic gene set for the intrinsic subtyping (Hollestelle et al. submitted for publication).
KK and ST were also involved in protein expression and purification.
In contrast to L3i and L3ir, most of the known L3c up-regulated genes were involved in protein expression, reflecting increased protein synthesis during culture (Table 6).
In another study [ 4], a gene involved in metabolism that was completely induced and hence effectively unregulated was shown to evolve to optimal expression levels, which could be predicted using measurements of the costs involved in protein expression [ 11, 12].
Recent studies have shown that patients with SLE have monocyte/macrophage defects involving surface protein expression, cytokine production, and phagocytic capacity, suggesting that monocytes and macrophages are key players in the pathobiology of SLE [ 10].
These results suggest that CR has a generally feminizing effect on gene expression, which involves protein metabolism and turnover, which might contribute to the lifespan extension effects of CR.
Now, Hsu et al. have shown that the Arabidopsis clock also involves proteins that increase the expression of certain genes at specific times of the day.
