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The concepts pursued involve annealing with low thermal budgets as well as defect engineering.
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Repair by the aNHEJ pathway often involves annealing of two partially complementary ssDNAs.
If Pif1 oligomerization is involved in annealing, then annealing activity should decrease if the Pif1 DNA ratio is decreased.
The distance-reactivity pattern for remotely aligned peptides was remarkably different to reaction scenarios that involved seamlessly annealed peptides with overhanging functional groups.
Flexible Latin hypercube sampling involves simulated annealing for optimally selecting accessible sites from a region.
(1) Since primers can anneal to DNA sequences with less than perfect complementarity and since this may potentially involve primer adenines annealing to unconverted non-CpG-cytosines, we omitted all MRED enzymes with sites within primer sequences.
These involve the interference with reverse transcription and with proviral integration by disturbing tRNA primer annealing and removal, DNA synthesis initiation and elongation and strand transfer reaction [ 43– 45, 126– 126].
The SDSA sub-pathway of DSB repair involves displacement of the newly extended strand from the D-loop followed by annealing with its original complementary strand.
Johnson, M. W. et al. Quantum annealing with manufactured spins.
Note that low temperature annealing with longer time sharpens the Tc transition and reduces the inhomogeneities.
This DNA chip format involves a target sequence which is annealed with a pair of labeled oligonucleotides (stabilizing oligonucleotides) prior to hybridization to the array of capture probes, forming a single-stranded gap between the stabilizing oligonucleotides and corresponding to the length of the capture probes.
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