A single investigator system of this nature, utilizing experienced independent investigators with years of specialized investigation and prosecution training, would offer victims and the accused the benefit of professional investigation without the delay and sometimes impersonal harshness of the criminal justice system.
This paper reports on a series of 55 oral histories with principal investigators, project managers, co-investigators, system engineers, and senior management from nearly every competitively selected Discovery mission launched to date that discuss the definition and evolution of these roles and share revealing insights from the key players themselves.
Stereological analysis and quantification of neocortical and hippocampal intra-neuronal FL-αsyn and CC-αsyn immunoreactivity was conducted by the disector method using the Stereo-Investigator System (MBF Bioscience, Williston, VT) and the results were averaged and expressed as cell counts per 0.1 mm3.
Sections were examined on an Olympus BX61 microscope and analyzed using the optical fractionator probe and a Stereo Investigator system with MicroLucida (MBF Biosciences).
Using the Stereo Investigator System (MicroBrightField Europe e.K., Magdeburg, Germany), an optical fractionator, stereological design was applied to obtain unbiased estimates of total Nissl-stained and FB-positive neurons.
Sandusky's statement that he was brought down by a "well-orchestrated effort of the media, investigators, the system, Penn State, psychologists, civil attorneys and other accusers," was also criticized by Cleland.
RFP-expressing beta-cells were captured using the Stereo Investigator Imaging System (SI).
RFP-expressing beta-cells were visualized using the Stereo Investigator Imaging System (SI, MicroBrightField, Williston, VT).
NeuN-positive cells were counted in all cornu ammonis (CA) fields and dentate gyrus (DG) in 2 sections through the anterior hippocampus and Iba-1 positive cells in the right hemisphere in 4 sections, using stereological principles (Stereo investigator 7, System Inc, Magdeburg, Germany), with a counting frame of 40×40 µm per section for NeuN, and 150×150 µm per section for Iba-1.
Depending upon the user access rights the user's are allowed to view only that part of the data for which principle investigator or system administrators have allowed access to a particular user.
Any treatment decisions requiring knowledge of the treatment allocation were referred to the data monitoring committee (DMC) or chief investigator, and systems were in place to allow for the breaking of the allocation code if required.
