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To obtain a list of putative solubly expressing clones for further investigation in the second liquid expression and purification step of the procedure, only colonies exhibiting visible hexahistidine tag signals were selected, thus eliminating truncated or degraded protein products that, even if soluble, would not be purifiable (Fig. 2).
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That's why I urged the investigation in the first place.
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Indeed, it was the chance discovery of apparent bias in face-tracking by one of the programs that prompted Buolamwini's investigation in the first place.
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