Another reason for investigating LINE-1 elements is its function.
One hour after the inoculation, and for each investigated line, around 50 100 mg of infected seedlings (only aerial parts) were transferred into a pre-weighed 1.5 ml tube containing 200 μl of 10 mM MgCl2 and 200 μl/L silwet.
Next, we investigated line-specific gene expression for ALVEs and TVB with real-time RT-PCR.
These include the frequently investigated lines, such as U87MG, SF-767 [ 22] and HSR-GBM1 [ 23].
In seedling assays 10 µM ABA inhibited growth of all investigated lines.
All investigated lines were homozygous for the Aβ transgene unless indicated by "1×Aβ", meaning flies were heterozygous for the transgene.
These FS changes seem to be rather specific for the reference genome as most other investigated lines have the obviously correct gene sequence (Table 4).
In order to delineate severity of local tissue inflammation, we investigated lining layer thickness, overall cellularity, density of CD3+ T cells, CD163+ macrophages, and vascularity.
The STRUCTURE software, in which SNPs with MAFs of >0.00 were included, was used to assess the genetic relationships of the investigated lines.
Analyses of the cpVenus173/YFP fluorescence emissions of the investigated lines indicated, that ABAleon2.1 (line 3) exhibited a ∼ fivefold higher fluorescence emission (expression) when compared to ABAleon2.1 (line 10) while emission of YFP-ABI1 was ∼ 7 % compared to YFP-PYR1.
On NIAS Genebank databases (http://www.gene.affrc.go.jp), 71 phenotypic traits are registered for 9 479 accessions (the numbers of investigated lines differ depending on the traits) as actual measured numeric data, qualitative data, and ranked data.
