The foraminiferal content was investigated under a binocular microscope and recorded semi-quantitatively.
Fluorescent change of the cells was investigated under a confocal microscope FV-10000; Olympus, Tokyo, Japan).
LPL expression in DPCs was investigated under a fluorescence microscope (Axiovert, Zeiss, Germany).
Cytospin smears were subsequently investigated under a light microscope (Axiostar plus, Zeiss Inc., Thornwood, NY, USA) and photomicrographs were taken.
The sections were then counterstained with hematoxylin, dehydrated, mounted with permount, and investigated under a light microscope.
Each vaginal sample was rehydrated with normal saline and investigated under a phase contrast microscope with 400 times magnification and with a microscopic area of 0.016 mm.
Shape and surface morphology of the SLNs confirmed spherical shape of nanoparticles when investigated under a transmission electron microscope.
Slides were investigated under a LEICA fluorescence microscope and digital images were obtained using a computer 'Cytovision Probe' (Applied Imaging System).
Thin sections were stained with lead citrate, and were investigated under a transmission electron microscope (Zeiss EM 900).
The stained cells were investigated under an Olympus BX50 microscope equipped with a Keyence VB-7010 CCDled colorolor camera and a VB-7000 digital microscope camera control system (Keyence Japan, Osaka, Japan) or Olympus FluoView 1000 confocal microscope.
