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In addition, we investigated template DNA amounts as low as 1 ng to evaluate the smallest amounts of genomic DNA samples that we could use successfully in our multiplex PCR methods for NGS-based HLA genotyping.
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Therefore, the results of the systematic mapping study could be interesting to researchers and practitioners who would like to propose a new SPL use case template or to investigate which template is better.
It should be noted that in initial experiments we investigated different templates T with various nucleotide gaps (0 4 nt) between the two annealing sites of L and R. When the two probes were separated by 0, 1, and 2 nt an active catalyst was formed; at greater distances (3 and 4 nt) dehalogenation did not occur (data not shown).
To investigate whether template size influences relaxivity, nanocages with one to four hydrophobic domains were designed.
To assess the relevance of the proposed technique, this paper first investigates the template ability to ensure the side information refinement.
To address this issue we analyzed SF using spiked SF samples and human peripheral blood monocytes (PBMO) persistently infected with C. trachomatis in vitro in serial dilutions to investigate which template preparation methods provide the best amplification substrate for each different assay type.
Such an approach was also reported by several authors, for example Yilmaz, Mosbach, and Haupt [ 53] who investigated how much template was actually needed to create a sufficient number of binding sites.
Of the templates investigated biphenyl ethanone was promising and led to inhibitors with IC50 values in the low micromolar range.
The optimal annealing temperature and time, surface morphology, and optical characteristics of the PSS-ANP template were investigated.
Mi et al. [9] realized AlN NW growth using a GaN NW template and investigated their optical properties.
The electrodeposition of Bi2Se3 nanowires on an anodic aluminum oxide template was investigated by cyclic voltammetry in a tartaric acid aqueous solution.
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