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DOI: http://dx.doi.org/10.7554/eLife.08760.013 Phase-locking analysis does not explicitly investigate OFF states, so we next automatically detected OFF states in electrodes that contained both MUA activity and TRN-induced slow waves.
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We sought to investigate off-line memory processing as directly as possible and therefore perfusion scanning was performed without concomitant functional challenge to direct or control subjects' mental engagement after learning (e.g. [43]), or during sleep [44], [45].
In order to further investigate off-line memory processing following the encoding of associative memories, we also adopted a pre-post learning study design from previous electrophysiological studies in experimental animals (e.g. [1]).
To investigate off-target effects, we chose the nematode, C. elegans, because of its resistance to lethality from AChE inhibition.
In future studies, we will investigate off-target effects of Chol-let-7a in preclinical animal models.
A major importance of this work is that it did not investigate off-target effects related to a specific therapeutic intervention.
It will be important to assess the effect of sActRIIB on other TGFβ family ligands for its specificity, as well as to investigate off-target effects of downstream signaling on other processes in which the TGFβ pathway is involved, before this decoy receptor can be developed as a viable treatment for cancer cachexia.
All children were investigated off medication.
Clarett, who set Ohio State freshman records for rushing yards and touchdowns as the Buckeyes won the national championship, missed most of the team's summer practices and its first two games while the university and the National Collegiate Athletic Association investigated off-the-field matters.
Off-target deamination could be a concern in base editing, so we further investigated off-target deamination in this HBB −28 (A>G) mutant cell line.
In the present study we directly investigated off-line processing of associative memory formation in the human brain.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com