In experiments investigating commitment to cell death after short-term khat exposure, 1 ml aliquots of treated cells were diluted in 10 vol. medium, and centrifuged (65 g av. for 5 min).
To investigate neuronal commitment of progenitor cells in the adult hippocampus, we used a knock-in mouse line expressing nuclear green fluorescent protein (GFP) under the control of the Tis21 promoter (Haubensak et al. 2004).
The purpose of this paper is to identify the major challenges to this call and investigate the commitments necessary to achieve this objective.
We established a suitable model for investigating MSC commitment and differentiation.
We first investigated the commitment of progenitors toward photoreceptor fates by detecting the homeobox protein Otx2, an essential positive regulator of photoreceptor production.
We also investigated the commitment to endothelial cells of MSCs, a naturally occurring stem cell population which resides in bone marrow and other tissues [ 25].
They were eager to investigate choice and commitment – their own as well as other people's.
Before jumping to the conclusion that the world is going to hell, it's probably a good idea to investigate these emerging commitment devices.
To investigate neuronal fate commitment, we used the marker neuronal class III β-tubulin (TuJ1).
To investigate which lineage commitment is predominant in fetal spleen, we performed hematoxylin and eosin staining at 16.5 dpc and 19.5 dpc.
