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The element is 2476 bp in length and encodes a single open reading frame (1.8 kbp) and terminates with 13 bp inverted terminal repeats (ITR).
ITR, inverted terminal repeat.
In addition, the genome contains inverted terminal repetitions (ITRs), which include a hairpin loop that connects the two strands of DNA.
rAAV gene transfer vectors were generated by inserting specific gene cassettes into rAAV2-based expression vectors, containing inverted terminal repeats of serotype 256, a woodchuck posttranscriptional regulatory element (WPRE), and a bovine growth hormone polyadenylation site (bGHpA).
For regulatable expression from the liver we designed a hepatospecific bidirectional and autoregulatory tetracycline (Tet -On sysTet -OntbidirAlb) flanked by AAV inverted terminal repeatsystems).
We propose that the spontaneous deletion and mobilization of vector sequence from the recombinant adenoassociated virus (rAAV) integration locus is mediated by a recombination event between the inverted terminal repeats that define the boundaries of the individual genome subunits.
These variants thus possess inverted terminal repeats (ITRs) from 21 to 50 kbp long; all are longer than the ITRs of CPV-BR (10 kbp).
Each end of the single-stranded DNA genome contains an inverted terminal repeat that is mandatory for replication and packaging.
The inverted terminal repeat (ITR) sequences for HAdV-D53 were determined to be 212 bp in length.
The 4.7 kb genome consists of two genes, rep (replication) and cap (capsid), flanked by 145 nucleotide (nt) inverted terminal repeats [ITRs, 1].
Another link between Slfns and responses to viral infections is suggested by the similarity between these genes and the right inverted terminal repeat of several orthopoxviruses [5], [35].
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