The slides were then examined under inverted fluorescence imaging.
GFP and mCherry reporter expression was observed and the various patterns were recorded under either a Nikon Eclipse 80i upright microscope or a Zeiss Axiovert inverted fluorescence imaging system, equipped with a Coolsnap monochrome camera (Photometrics, EZ).
The fact that the membrane is transparent introduces the possibility of wide field inverted optical imaging to brain slice electrophysiology.
Adult worms were kept in cohorts to determine age and examined using inverted fluorescence imaging without anaesthetics.
At 24 hours following essential oil treatment, images of multicellular spheroids were visualized and captured with the Nikon inverted microscope and imaging software.
Two days after transfection, the cells were preincubated at low glucose for 30 min. The coverslips were then mounted in an open perfusion incubator at 37°C on the stage of a Zeiss Axiovert 200 fluorescence inverted microscope modified for imaging experiments [39].
Mitotic chromosomes were viewed with a Leica DMI6000B inverted microscope and fluorescence imaging workstation equipped with a HCX PL APO 100×/1.4 0.7 oil objective.
The interpreted result shows that the trained network was able to invert 2D electrical resistivity imaging data obtained by a Wenner Schlumberger configuration rapidly and accurately.
Live imaging was performed with a Zeiss Axiovert 200 inverted microscope and images taken and processed with the Improvision software Openlab 5.0.1.
