The contact potential difference maps measured on the PS and the PMMA films show inverted contrast.
NK cell-mediated killing of solid tumor cell lines was visualized by life imaging on a Zeiss Axiovert 35 M inverted contrast microscope (Zeiss, Sliedrecht, The Netherlands) which was placed in a 37°C incubator.
Under inverted contrast microscopy, LM8 cells were polygonal with a full cytoplasm.
The growth of LM8 cells on type I collagen plaques was monitored under an inverted contrast microscope.
(B,E,H) Inverted contrast illustrations of Rab9-GFP in the MDCK cells shown in A, D and G. (C,F,G) Merged color illustrations of the images shown in A-B, D-E and G-H, respectively.
The deletion of both pmcA and pmcB additionally results in vacuolar fragmentation and such fragmentation occurs irrespective of the halA and sltA alleles carried (Fig. 4, inverted contrast inset).
Rigid registrations with BSpline interpolation were performed, using Elastix C++ [ 28], to register (i) the T2 maps to the T1 maps (MP2RAGE) and (ii) the T2 ∗ maps, MPRAGE, FLAIR, and DIR images to one of the inverted contrasts of the MP2RAGE sequence.
We used the Elastix c++ library 33 to perform (1) rigid registration with BSpline interpolation of the T2 maps to the T1 maps (from the MP2RAGE); and (2) rigid registration of T2* maps, MPRAGE, FLAIR, and DIR images to one of the inverted contrasts of the MP2RAGE sequence.
Following correct responses (i.e., correct classification of the image orientation as upright or inverted), mask contrast was increased by 0.05, and following incorrect responses, it was decreased by 0.05 (i.e., 1-up, 1-down staircase procedure61).
1.5 × 103 cells were plated in 24-well plates (day 0), treated with the various drug combinations after 24 h, and counted at days 1, 2, 4, 7, 10, and 14 by using both a hemocytometer and a Leica S80 inverted phase contrast microscope, as well as the Countess Automated Cell Counter (Invitrogen).
For fluorescence images, the figure panels were assembled as a single image then inverted and contrast adjusted evenly for better visualization.
