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Three of these inversions (Inv1n, Inv4m, and Inv9d) showed altitudinal clines (supplementary fig. S8, Supplementary Material online), though the signal at Inv1n was less clear than originally reported in Fang et al. (2012) as the derived (inverted) arrangement was absent from the low elevation Crucero Lagunitas population of parviglumis.
The inverted arrangement places the two composite active sites in each subunit on opposite lateral sides of the homodimer.
For example, Adh and bib were shown here to have 0.6% and 3.3% recombinants respectively with the centromere, but none were observed among a similar number of progeny when the X-4 fusion had the inverted arrangement.
PCR across the junctions of the inverted arrangement was attempted with a pair of distal primers (D1F, 5'-GCGTTGTCAATAATGCCTGA-3'; P3ST, 5'-CGAAGAGGAAGTCGTGCTTT-3'), and a pair of proximal primers (D2ST, 5'-GGCGGATTCTAGCAAATGTC-3'; P4U, 5'-GTTGACGTTTTGGGTCGTTT-3').
To isolate the AC and BD regions of the inverted arrangement, two 2jz3 lambda genomic libraries were screened with appropriate probes from regions C, D and B (see Materials and Methods).
Contig GCTO4WP02F0TDF.f.pc. 1indicates that Pycnoporus is another species with this same inverted arrangement.
Similar(37)
This unexpectedly high level of conservation and the closely linked and inverted physical arrangement of the two genes (which is reminiscent of many other examples of unexpected nucleotide conservation in duplicated genes in the literature) is consistent with a proximity-based gene conversion mechanism.
A conventional tubular absorber is modified by introducing film-guiding fins between tubes to produce a film-inverting arrangement.
We then annotate them by comparison with the genome of D. virilis, the closest relative with a sequenced genome [ 35] that represents the ancestral (non-inverted) arrangement.
Both these models predict that the genetic content of inverted and standard arrangements should differ, and that differences may also develop within arrangements at different sites.
For the purpose of analysis, inverted and standard arrangements were treated as alternative alleles of a bi-allelic locus.
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