Sentence examples for inversion reaction from inspiring English sources

Each inversion reaction was initiated by the expression of one of the recombinases, FimB or Hin.

The present postulated inversion reaction mechanism of enzymatic hydrolysis in CelS involves a proton transfer from the acid catalyst (Glu87) to the leaving group O4 and a nucleophilic attack by a water molecule at the anomeric center (C1) assisted by the base catalyst (Asp255).

Fis-Hin connections at early and late steps in the DNA inversion reaction.

Hin-catalyzed DNA inversion reaction and outline of Fis-Hin crosslinking experiments.

The linearized probe has undergone an inversion reaction and it can now serve as template for subsequent amplification reactions.

We conclude that basic residues in the Hin helix-B region function in the Fis/enhancer-dependent activation step of the Hin-catalyzed DNA inversion reaction.

Secondly, Flp catalyzes intramolecular excision or inversion reactions on DNA molecules containing two FRT sites as well as intermolecular reactions between molecules containing FRT sites [ 7].

In vitro DNA cleavage and inversion reactions were performed as previously described (Haykinson et al., 1996) using pMS551 (unless otherwise stated), which contains the native hixL-enhancer spacing.

Inversion reactions were performed in the presence and absence of Fis for 0, 1, 2, and 5 min. Hin-R48A/K51A exhibits 10-fold slower kinetics than Hin-wt under Fis-activating conditions, but is fully competent for Fis-independent inversion when coupled to the hyperactivating mutation Hin-H107Y.

In order to identify inverted alleles, we performed a final inversion PCR reaction using one external and one inverted internal primer, as shown in Additional file 4: Figure S4A (primers in Additional file 6: Table S6).

The TST calculations of the rate constants and their temperature dependence for the direct and reverse "inversion substitution" reactions have been carried out within the temperature interval of 273 2000 K.