Sentence examples for inverse polymerase from inspiring English sources

inverse polymerase chain reaction.

We describe methodologies for the rapid, efficient, and economical construction of such libraries using inverse polymerase chain reaction (PCR).

A long-distance inverse polymerase chain reaction (PCR) using nested primer combinations designed for each constant gene of IGH showed that Cγ4 was juxtaposed to the downstream sequence of the BCL2A1 (BCL2-related protein A1) gene through the Sγ4 switch region.

Insertion sites were determined by inverse polymerase chain reaction (PCR) (8).

adjacent to the 1B4 provirus were isolated by inverse polymerase chain reaction (PCR) as reported elsewhere [ 29].

Inverse polymerase chain reaction (PCR) was used to clone the genomic insertion site of the R11 promoter trap vector.

The position of the transposon was determined by inverse-polymerase chain reaction [31], sequencing of the amplicons, and comparison to the available sequence of P. aeruginosa (http://www.pseudomonas.com/).

An inverse-shifting polymerase chain reaction (IS-PCR) combined with short-end capillary gel electrophoresis (CGE) was developed for genotyping of intron 22 inversion Type 1 (Inv22-1) and Type 2 (Inv22-2) of hemophilia A (HA).

By using site-directed mutagenesis with Vent polymerase and inverse PCR, charged or polar residues (including His, Lys, Arg, Asp, Glu, Ser, Thr, Gln, and Asn) were individually mutated to Ala one residue at a time, and each Tyr residue was mutated to Phe.

The mitochondrial genome has a length of about 2 kb and is circular, as demonstrated by polymerase chain reaction (PCR) and inverse PCR.

NC_000913) was synthesized in the primers ex-N and ex-C (Table 4), and introduced into pHsh by an inverse PCR using the high-fidelity Pyrobest DNA polymerase (TaKaRa); PCR product was phosphorylated and self-ligated to generate new vector pHsh-ex (GenBank accession number of FJ715939).