Interestingly when we introduced antisense PCDGF into less invasive SKov3 cells, cell proliferation rate and invasion index decreased slightly without statistical significance (data not shown), which might indicate that antisense PCDGF worked more efficiently in highly invasive ovarian cancer cells.
To evaluate the invasive properties, without accounting for the intrinsic migration properties, we calculated the invasion index by dividing the percentage of cells invading through Matrigel-coated inserts with the percentage of cells migrating through non-coated insert.
As shown in Figure 1D, transfection of melanoma lines with synthetic miR-200c resulted in either no change in invasion index in the Wm266.4 cell line, or a 2-fold activation of invasive capacity in Wm1361 and Sbcl2.
Results of invasiveness and angiogenic potential were normalised with vehicle-treated cells and expressed as invasion index.
SW620, LoVo and DLD-1, HCT15 have shown the highest and lowest Invasion Index, respectively.
Normal mucosal cells CCD841 CoN showed an Invasion Index of 0 (Fig. 2).
Our study confirmed that SW620, LoVo and DLD-1, HCT15 have shown the highest and lowest Invasion Index, respectively.
The percentage of cells that have migrated through the membrane was calculated and was recorded as the Invasion Index.
SW620 and LoVo had the highest Invasion Index whereas DLD-1 and HCT15 had the lowest one.
Based on results of the invasion study, two cells with the highest Invasion Index and the two least aggressive cells were selected to test their Mn uptake at MRI, using normal cell CCD841 CoN as control.
In this assay, the relative migration of cells from the upper chamber to the lower chamber compared with a reference cell line (non-tumorigenic 10-87 Lprovidesdes an invasion index.
