These universal positions covered the whole range between invariable, moderately variable, and highly variable characters.
As I watched the priests and their attendants and became familiar with the invariable and variable parts of the great variety of Masses that are celebrated in these churches, some forgotten observations and speculations on the Mass that I had read through the years in books on archeology and anthropology and related subjects began to return to my mind.
The general time-reversible model, with some sites assumed to be invariable and variable sites assumed to follow a discrete gamma distribution (GTR + I + Γ; [ 33]), was selected as the best-fit model of nucleotide substitution by MrModeltest 2.2 http://www.abc.se/~nylander/[ 34].
Of those 2101 characters, 1889 were invariable, 212 were variable, and 160 of the variable characters were parsimony informative.
Within the genus Crenadactylus 380 sites were invariable, 32 were variable but not parsimony informative, and 416 were variable and parsimony informative.
The combined dataset (CAL + ITS) yielded a sequence alignment of 1,397 positions, including 866 invariable characters, 232 variable parsimony-informative sites (16.6%), and 185 variable singletons.
The variable, invariable and parsimony-informative sites in these alignments were identified using DnaSP.
Aligned sequences of CAL were 729 bp long, including 355 invariable characters, 216 variable parsimony-informative (29.6%), and 133 singletons.
The aligned CAL sequences were 595 bp long, including 302 invariable characters, 239 variable parsimony-informative sites (40.17%) and 43 singletons.
The aligned ITS sequences were 506 bp long, including 407 invariable characters, 85 variable parsimony-informative sites (16.79%) and 12 singletons.
