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Unique substitutions were counted at sites that were invariable in all but one or two sequences, that is, sites clearly under strong stabilizing selection but still capable of at least some change.
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A general time-reversible (GTR) model of nucleotide substitution with the ML estimates for gamma distributed rate heterogeneity of four categories (Г4) and a proportion of invariable sites were used in all tree reconstruction methods.
gambiae chromosomes and colocalized with the HP1 antibody in all major invariable sites and in most of the variable sites.
The WAG amino acid substitution model [ 92] with gamma distributed site rates and an invariable site category was used in all phylogenetic analyses as the Prottest software [ 93] indicated that this model provided the best fit to the data.
gambiae chromosomes with antibodies against Drosophila melanogaster heterochromatin protein 1 (HP1) and the nuclear envelope protein lamin Dm0 identified the major invariable sites of the proteins' localization in all regions of pericentric heterochromatin, diffuse intercalary heterochromatin, and euchromatic region 9C of the 2R arm, but not in the compact intercalary heterochromatin.
van de Laar et al. found the invariable presence of osteoarthritis at a young age in all patients with SMAD3 mutations.
Nucleotide heterogeneity of substitution rates, estimated with a gamma distribution (G) was included in all cases whereas a proportion of invariable sites (I) was found appropriate for 9 of the 16 partitions (data not shown).
All in all, the show emphasizes Mr. Wegman's invariable lightness of being as an artist, his penchant for making the most of as little as possible and doing it as transparently as possible.
Further PCR analysis confirmed the invariable insertion of tetO into the same chromosomal locus (cjsa_0191) in all clone SA isolates collected since 2003 (over 100 isolates), all of which are resistant to tetracyclines (minimum inhibitory concentration [MIC] ≥16 µg/ml).
Imprinted genes associated with invariable DMRs are likely to be targeted for demethylation, and imprinted, in all strains.
A second key pertinent issue is whether the same benchmarks are appropriate at all levels and in all settings, on the basis that quality is an invariable construct.
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