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These included 322 valid samples, 28 flagged samples due to sampling times being 12.5 to 25% greater or less than the targeted 24-hours, 24 invalid samples due to sampling times being beyond the targeted 24-hours ± 25% or due to damage of the sampler, and 1 sample below detection.
Through Facebook and the PTT Bulletin Board System, 466 online questionnaires were returned, 11 of which were deemed to be invalid samples.
In using the above equation, measurement samples Y k ( off ) ( n ) = 0 would have been treated as invalid samples and discarded from the result to avoid division by zero, though in practice, such samples did not occur during the experiments that were made.
Invalid sample assays were only 0.4%.
Data from invalid sample assays were excluded from statistical analysis.
Of the invalid samples (without amplifiable DNA), 75% came from males; males were twice as likely as females to submit invalid samples.
The only invalid sample on THxID™-BRAF presented a low rate of necrosis.
The single invalid sample obtained with THxID™-BRAF was found positive for V600E using HRM and Sanger sequencing.
> -wrap-foot> Adequate/valid samples were samples returned in an aluminium bag with a desiccant pouch while invalid samples were samples without a dessicant pouch or aluminium bag.
The frequency of invalid samples and the methylation status of the samples were not dependent on the study centre where the biopsy was taken (P=0.11).
Further, women with an adequate/valid sample were more highly educated and healthier than those who did not participate or had an invalid sample.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com