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The presence of an endothelial barrier does not prevent invasion of PC-3 cells towards BMS); however, there is a marked increase in the number of PC-3 cell invading towards TCP with significantly similar numbers invading towards both TCP and BMS stimuli (136±32 and 107±9 P=0.4988).
A minor change in CI was recorded when HCT116 cells were invading towards DMEM or media derived from HCT116 cultures indicating that little or no invasion and directional cell migration through the matrigel layer occurred.
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Treating PC-3-GFP cells with either GGPP or mevalonate had no significant effect on their ability to invade towards BMS (P=0.259 and 0.619, respectively).
Figures 5D and E show that high levels of EphA2 receptor expression correlated with malignancy and the ability to invade towards AA.
Cells suspended in serum-free media were added to each chamber and allowed to invade towards the underside of the chamber for 20 h at 37 °C.
The MCM was used as a chemoattractant in the lower chamber of the Transwell and SCC cells were allowed to invade towards this stimulus for 72 h before being counted.
As observed in the PC-3 cell lines, 1 μ M SIM did not affect the ability of DU-145 to invade towards AA through a Matrigel barrier but it significantly reduced TEM across a BMEC layer.
Using validated human BMS co-culture models (Hart et al, 2005), we have shown that lipophilic statins all affect the ability of PECs to invade towards and through BMS to initiate and develop tumour colonies.
Loss of GGPP leads to a significant reduction in the ability of malignant PEC to invade towards and through BMS and so reduce their ability to form colonies within the BMS.
Primary prostate epithelial cells had a weaker effect on the BMECs, inducing fewer endothelial cells to invade towards TCP, with only BPH cells stimulating significantly more endothelial cells to invade (73±8 vs 34±11; P=0.037) than the no prostate epithelial cells control.
4 T1 or PyV MT cells in serum free media (50,000 cells) were plated over transwell inserts (BD Biosciences, San Jose, CA), pre-coated with reduced growth factor matrigel (BD Biosciences, San Jose, CA, USA) and were permitted to invade towards lung and bone lysates (500 ug protein) contained in the bottom chamber for 24 hours.
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Justyna Jupowicz-Kozak
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