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The ratio of invading cells over non-invading cells was compared between the various treatments.
Non-invading cells were removed, and invading cells were fixed with methanol for 5 min and stained with 0.1% crystal violet for 15 min before imaging and quantification.
When incubated in a mixture of Matrigel and culture medium non-invading cells formed spheroid cultures, whereas invading cells showed a stellate outgrowth.
Non-invading cells were removed using a cotton swab, and invading cells on the underside of the filter were counted with an inverted microscope.
Non-invading cells on the top side of the membrane were removed, while invading cells were fixed and stained with 0.1 % crystal violet, 24 h post-seeding.
Sentries called dendritic cells lie in wait at portals of entry, like the skin, ready to devour invading cells.
However, a secondary, long term, aim is to provide invading cells with a matrix for attachment (Lawrence 1998).
It does so by invading cells lining the intestines, leaving blood cells untouched.
The researchers still can't see nanoscale processes like viruses invading cells in living tissue samples.
Invading cells were enumerated under light microscopy.
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Moreover, SP600125 cotreatment did not change the number of membrane-invading cells.
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