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Five months later, on June 25 , 1950 the North Koreans invaded across the 38th parallel.
Today marks the 60th anniversary of the Korean war, which began when the North invaded across the 38th parallel.
Israel invaded across the Blue Line that marks its border, then occupied a swath of the south.
The Celts' nemesis, the Anglo-Saxons, also invaded across the sea, landing here with such ferocity that, according to the chronicle, the Britons fled westwards "as if from fire".
Cells which invaded across the membrane were stained with hematoxylin and eosin stain and counted (Fig. 4A B).
Using a Matrigel invasion assay that mimics active invasion of tumor cells across a basement membrane, we found that after 48 hours, the number of C2GnT1 shRNA-transduced LS174T cells that invaded across the Matrigel membrane was significantly decreased compared to LS174T cells transduced with scrambled shRNA (mean number of cells/field = 27 versus 162, p<0.0001) (Fig. 6A, C, and D).
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The B. cenocepacia environmental isolates (n = 35) survived in the hypodermic air pouch but did not invade across the peritoneal epithelial layer during a 72-h infection.
These observations suggest that this model may allow the investigation of cells as they invade across the BM.
This model potentially generates a three-stage assay that allows investigation of cells: i) contained by BM; ii) as they invade across BM; iii) as they invade more distally into surrounding collagen.
By day 7, the HBL100 and HS578T cells have reverted to an elongated morphology, indistinguishable from that seen in collagen, and are invading across BM and into surrounding collagen.
First, as noted earlier, it supports the view that vector-transmitted plant pathogens such as X. fastidiosa rarely invade across geographical barriers otherwise we would not be detecting such unique invasions.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com