Meanwhile, large multi-centre studies — e.g. the Microarray Innovations in LEukaemia (MILE) study 10 — prepare for a standardised introduction of gene expression profiling in diagnostic algorithms, aiming to translate this novel methodology into clinical routine for the benefit of patients with the complex disorders.
Specificity was indicated by successful partial rescue following introduction of an expression construct of C3 resistant to the shRNA (Fig. 3d,g).
The introduction of prokaryotic expression elements and the precise design of the compatible RBS and Kozak sequence endow pOmni with pro/eukaryotic dual-expression capability, which was validated by cloning and expression of reporter gene EGFP in E. coli and HeLa cells in this study.
It is known that through the introduction of an expression vector into E. coli or other hosts for recombinant protein production, the native cell functions at many levels can be perturbed (e.g. ribosome functions, RNA turnover as well as energy and intermediary metabolism of the cell) (Bailey 1993; Hoffmann et al. 2002; Lin-Chao et al. 2006).
Introduction of exogenous expression of the rovA gene under the control of the arabinose-inducible promoter araBADp into the mutant strain restored RovA expression when induced with arabinose.
Since the introduction of gene expression microarrays in the mid-1990s, genome-wide exprofilingprofiling has been widely utilized in cancer research [63], [64].
The introduction of cloning expression methodology [4], [5] has led to the successful cloning of a glycosyltransferase and to the demonstration that overexpression of a glycosyltransferase cDNA clone can confer the capability of glycan biosynthesis in overexpressing cells [6].
A low basal production of PGF2α was detected in similar amounts in three independent COX2-expressing clones while a 5- to 20-fold increase of PGF2α levels was observed in three independent clones upon stable introduction of AKR1B7 expression vector.
In addition, they contain Gateway™ recombination sites for high-throughput introduction of shRNA expression cassettes after in vitro recombination.
Here we describe the introduction of an expression cassette carrying the α-AI1 gene under phytohemagglutinin seed-specific promoter control in C. arabica plants using biolistics, followed by regeneration of coffee plants.
