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Finally, the active catalyst was also generated in situ by pre-mixing the gold chloride complex with the silver additive prior to addition of the substrate, or by introducing an intermediate operation, in which the AgCl formed was removed by filtration through a bed of Celite before the introduction of the substrate.
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This is best evidenced in Figure 4 where glutamine, aspartate, serine and glycine do not become noticeably labeled until 40 h after the introduction of the labeled substrate.
Simultaneous to the introduction of the labeled substrate, subsets of both unlabeled and labeled cultures were treated with LPS (added to culture media to a final concentration of 1 μg/mL) for the duration of the labeling protocol.
Rather than administering excitation light, bioluminescence imaging based on firefly luciferase (luc) requires introduction of the enzyme substrate luciferin that reaches target areas via the blood supply and diffusion.
The substrate concentration in the measuring cell was specified by the introduction of aliquots of the substrate standard stock solution to the working buffer.
Electrochemical characterizations demonstrate that the introduction of LDH substrate can enhance the electrocatalytic performance toward H2O2 of the sensor.
Introduction of the protonphore caused substrate removal efficiency to decrease from a consistent value of 96%too 68.5% with considerable variance.
Cellulase recycling approaches have included recovery of free enzymes from the liquid fraction [ 36] and introduction of fresh substrate to the liquor fraction, allowing the enzymes to be absorbed to the substrate before separation and hydrolysis [ 37].
The introduction of hard substrate might lead to higher structural heterogeneity around the turbine foundations and the scour protection serving as artificial reefs, with an increasing habitat quality for prey too (Gordon 2012; Lindeboom 2012; Scheidat et al. 2011; Thompson et al. 2013; Winter et al. 2012).
The introduction of carbon substrates improves the electrical conductivity and stability of pFeMOF.
(29) It is notable that introduction of the Ala-Ala dipeptide substrate between terminal catechol groups and the PEG core did not significantly impact cAAPEG gelation behavior when compared to peptide linker-free catechol-modified cPEG adhesive hydrogel.
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