Sentence examples for introduction of sequences from inspiring English sources

The pk5 protein was obtained readily by the introduction of sequences recognized by protease factor Xa at the fusion site and ion-exchange chromatography was employed to purify pk5.

This strategy allows identification of translocation signals, as the introduction of sequences encoding the carboxy terminus of the previously uncharacterized protein Lpg1798 rescued the translocation defect of SidCΔ100.

TEs can cause human disease by creating insertional mutations in genes, and also contributing to genetic instability through non-allelic homologous recombination and introduction of sequences that evolve into various cis-acting signals that alter gene expression.

The typical approach involves the introduction of sequences of evolutionary interest into a model system, and then searching for spatial or temporal differences in gene expression that can be ascribed to the introduced sequence (Enard et al., 2009; Cotney et al., 2013).

This includes introduction of sequence diversity at two hinge residues, which connect the new loops with the rest of the protein scaffold, and we demonstrate that this is necessary for a successful exploration of functional sequence space.

The second CFTR allele of iPSCs from heterozygous donors or compound heterozygotes can be fully inactivated through introduction of sequence-specific deletions or transgene integrations utilizing designer nucleases such as TALENs or CRISPR/Cas.

Here we show how the introduction of sequence heterogeneity using a simple scheme of just two types of amino acids, hydrophobic (H) and polar (P), and sequence design allows a selected putative native fold to become the free energy minimum at low temperature.

In some cases, sequences were curated by introduction of sequence gaps to extend recognizable homology with the query sequence.

Especially before the introduction of sequence-based approaches pulsed field gel electrophoresis (PFGE) used to be the "gold standard" of MRSA typing [ 10].

For each PCR step, a high-fidelity DNA polymerase with 3ʹ−5ʹ proofreading ability and high processivity was used to reduce the introduction of sequencing errors.

The 'goodness' of the alignment can, thus, be estimated using the following χ scoring function: The introduction of sequencing errors complicates the matching process as real restriction sites may disappear from sequences and false ones may be created.