Sentence examples for introduction of restriction from inspiring English sources

Note that in both recombinant HrcUXAC_207-357 (through which HrcUXAC_207-264 is purified, see below) and HrcUXAC_207-357 AAAH), residues Gln207 and His208 have been mutated to Met and Asp residues, respectively, due to the introduction of restriction sites used in the cloning protocol.

Lowercase letters: deviations from the original OsRpl6-1 sequence to enable introduction of restriction sites.

This way of construction allows for fusion of protein encoding fragments without the introduction of restriction sites between the fragments.

Public health investigations of infectious diseases have relied increasingly on molecular epidemiology since the introduction of restriction fragment length polymorphism (RFLP) analysis in the 1980s.

In our work, we optimized the protocol such that only one set of primers are used to both amplify the genes and introduction of restriction sites, thus reducing the number of primers by half.

Polymorphism genotyping was performed by means of three techniques: (a) polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis, to identify the APOB p.E4181K, FABP2 p.A55T, LDLR c.1959C>T, and LPL p.S474X sites; (b) artificial introduction of restriction sites (PCR-AIRS), to detect APOA5 p.S19W and MTTP −493G>T; and (c) sequencing, for the LIPC −514C>T site.

Monkeypox virus infection of African rodents imported for the pet trade led to cases of the disease in pet prairie dogs and humans, resulting in the introduction of restrictions on the pet prairie dog trade.

Introduction of restrictions for 170 high-cost send-out tests resulted in a 23% decline in order volume.

Subsequent reductions in Sri Lanka's suicide rates have been attributed to the introduction of restrictions on the availability of highly toxic pesticides.

The introduction of restrictions on access to materials that have already been disseminated and have found widespread use does not add to the credibility of regulatory bodies.

Lebedenko et al reported an in vitro exon ligation method, but it involved complicated steps including two-step PCR to amplify the exons, cloning of all exons separately, introduction of specific restriction enzymes and ligation of multiple restriction fragments[25].