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It is based on the introduction of overlaps between the vector and insert by PCR.
However, nearly all current cloning methods still require the introduction of overlaps by PCR, which can introduce undesired mutations.
Eisenman is an artist of overlapping sincerities.
Separation of overlapping partials.
We are increasingly living in a time of overlapping spaces, and overlapping identities.
Under these conditions, the differentiation of the solid and liquid states becomes ambiguous, motivating the introduction of the term "overlapping" regime to describe this "twilight zone" NP state.
The evaluation of UniRef clusters and the evaluation of UniRef50-based sequence similarity search (Section 3.3) was conducted using UniRef release 2013_02 (February 6 , 2013 following the introduction of the 80% overlap threshold.
For example, the introduction of fillets at the overlap ends or tapering of the substrates can reduce the peel stresses at the overlap end.
It overlaps the introduction of the first referral guidance for suspected cancer sent to GPs in 2000, and does not take account of the minor changes when these were revised in 2005 [ 8].
With the introduction of disclination angle and overlap angle into graphene sheets, the resultant helical cones have variable morphologies which rationalize the broad distribution of the f-CNF apex angles.
The expansion of the higher educational sector in the United Kingdom over the last two decades to meet political aspirations of the successive governments and popular demand for participation in the sector (the Widening Participation Agenda) has overlapped with the introduction of e-learning.
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CEO of Professional Science Editing for Scientists @ prosciediting.com